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| Registros recuperados: 14 | |
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| Rosado,Tatiana Barbosa; Tomaz,Rafael Simões; Rocha,Rodrigo Barros; Rosado,Antônio Marcos; Alves,Alexandre Alonso; Araújo,Elza Fernandes de; Alfenas,Acelino Couto; Cruz,Cosme Damião. |
| The objective of this work was to verify the existence of a lethal locus in a eucalyptus hybrid population, and to quantify the segregation distortion in the linkage group 3 of the Eucalyptus genome. A E. grandis x E. urophylla hybrid population, which segregates for rust resistance, was genotyped with 19 microsatellite markers belonging to linkage group 3 of the Eucalyptus genome. To quantify the segregation distortion, maximum likelihood (ML) models, specific to outbreeding populations, were used. These models consider the observed marker genotypes and the lethal locus viability as parameters. The ML solutions were obtained using the expectation‑maximization algorithm. A lethal locus in the linkage group 3 was verified and mapped, with high confidence,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eucalyptus; Gametic selection; Genetic mapping; QTL; Rust resistance; Segregation distortion. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000900008 |
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| Costa,Leonardo Emanuel de Oliveira; Queiroz,Marisa Vieira de; Borges,Arnaldo Chaer; Moraes,Celia Alencar de; Araújo,Elza Fernandes de. |
| The common bean is one of the most important legumes in the human diet, but little is known about the endophytic bacteria associated with the leaves of this plant. The objective of this study was to characterize the culturable endophytic bacteria of common bean (Phaseolus vulgaris. leaves from three different cultivars (Vermelhinho, Talismã, and Ouro Negro) grown under the same field conditions. The density of endophytic populations varied from 4.5 x 10² to 2.8 x 10³ CFU g-1 of fresh weight. Of the 158 total isolates, 36.7% belonged to the Proteobacteria, 32.9% to Firmicutes, 29.7% to Actinobacteria, and 0.6% to Bacteroidetes. The three P. vulgaris cultivars showed class distribution differences among Actinobacteria, Alphaproteobacteria and Bacilli. Based... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endophytic bacteria; Common bean; Phaseolus vulgaris; 16S rDNA; Diversity indices. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400041 |
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| Varavallo,Maurilio Antonio; Queiroz,Marisa Vieira de; Lana,Taís Guimarães; Brito,Admilson Toscano Ribeiro de; Gonçalves,Daniel Bonoto; Araújo,Elza Fernandes de. |
| Protoplast fusion between complementary auxotrophic and morphological mutant strains of Penicillium griseoroseum and P. expansum was induced by polyethylene glycol and calcium ions (Ca2+). Fusant strains were obtained in minimal medium and a prototrophic strain, possibly diploid, was chosen for haplodization with the fungicide benomyl. Different recombinant strains were isolated and characterized for occurrence of auxotrophic mutations and pectinolytic enzyme production. The fusant prototrophic did not present higher pectinase production than the parental strains, but among 29 recombinants analyzed, four presented enhanced enzyme activities. The recombinant RGE27, which possesses the same auxotrophic and morphologic mutations as the P. griseoroseum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Protoplast fusion; Pectinases; Penicillium. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100011 |
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| Rocha,Rodrigo Barros; Barros,Willian Silva; Muro-Abad,Júpiter Israel; Tomaz,Rafael Simões; Cruz,Cosme Damião; Barros,Everaldo Gonçalves de; Araújo,Elza Fernandes de. |
| Características oligogênicas de distribuição discreta e expressão governada por poucos genes de maior efeito têm se mostrado importantes na condução dos programas de melhoramento, com destaque para a resposta de resistência das plantas às doenças. Métodos tradicionais de detecção de QTL's, que pressupõem normalidade e herança governada por múltiplos fatores, não deveriam ser utilizados para mapeamento dessas características de distribuição discreta e interação epistática predominante. O objetivo deste trabalho é avaliar os resultados de um método para mapeamento e detecção de locos controladores da expressão de características oligogênicas, OTL's (Oligogenic Trait Loci). Esse método, definido como MMCO (Método de Mapeamento de Características... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mapeamento genético; Locos controladores de características oligênicas (OTL's). |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000200012 |
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| Kasuya,Maria Catarina Megumi; Coelho,Irene da Silva; Campos,Daniela Tiago da Silva; Araújo,Elza Fernandes de; Tamai,Yutaka; Miyamoto,Toshizumi. |
| Eighteen Pisolithus basidiomes were collected from Eucalyptus plantations in the state of Minas Gerais, Brazil. These basidiomes were characterized morphologically and molecularly. The basidiomes varied in shape, color and size. One of them was found underground, indicating a hypogeous fungus. The main morphological distinctive characteristic was spore ornamentation, which distinguished two groups. One group with short and erect spines was identified as Pisolithus microcarpus, and the other with long and curved spines as Pisolithus marmoratus, after analyzing the cladogram obtained by phylogenetic relationship based on internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA of these isolates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ectomycorrhizal fungus; Phylogenetic analysis; Internal transcribed spacer (ITS); Nuclear ribosomal DNA; Taxonomy. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000600013 |
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| Coelho,Irene da Silva; Queiroz,Marisa Vieira de; Costa,Maurício Dutra; Kasuya,Maria Catarina Megumi; Araújo,Elza Fernandes de. |
| The aim of this work was to study the standardization of conditions to obtain and regenerate Epulorhiza repens and Ceratorhiza sp. protoplasts. For E. repens, the largest number of protoplasts (8.0 × 10(6) protoplasts/mL) was obtained in 0.6 M KCl, using 15 mg/mL of Lysing Enzymes, and 2-day-old fungal mycelium. When 0.5 M sucrose was used as osmotic stabilizer, the highest frequency of regeneration was achieved (8.5 %); 80.0 % of protoplasts were nucleated, and 20.0 % anucleated. For Ceratorhiza sp., the largest number of protoplasts (4.0 × 10(7) protoplasts/mL) was achieved in 0.6 M NaCl, when 15 mg/mL of Lysing Enzymes and 15mg/mL of Glucanex, with 2-day-old fungal mycelium were used. The highest frequency of regeneration was 6.7 % using 0.5 M sucrose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ceratorhiza; Epulorhiza; Molecular biology; Protoplast; Regeneration. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100019 |
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| Registros recuperados: 14 | |
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